Fig. 5.

THC suppressed the nuclear localization of NF-κB in LPS-stimulated BV2 microglial cells. (A) BV2 microglia cells were pretreated with THC for 1 hr prior to stimulation with 200 ng/ml LPS for 1 hr. Localization of NF-κB p65 subunit was determined using an anti-p65 antibody and an Alexa 546-labeled goat anti-rabbit IgG antibody. Nuclei were visualized by Hoechst staining. Cells were visualized using confocal scanning microscopy. Scale bar=20 µm. (B) NF-κB localization was further examined by line scannin.