Table 3.
Putative glycosyltransferase genes of H.somni strains
| Strain 2336 locus tag, protein,1(SSR, if present)n | Strain 129Pt locus tag, protein,1(SSR, if present)n | SWISS-PROT accession number | Closest Pasteurellaceae homolog Bacterium, locus tag, protein size,2identity | Closest GT homolog (Outside Pasteurellaceae) locus tag, protein,2identity |
|---|---|---|---|---|
| [GenBank:HSM_0148], 260 aa, (G)9 | [GenBank:HS_0275], 259 aa, (G)6 | [Swiss-Prot:B0UVK9/Q0I1L8] | None | Acinetobacter johnsonii, [GenBank:EEY94992], 281 aa, 44% |
| [GenBank:HSM_0164], 522 aa, (G)9 | None | [Swiss-Prot:B0UVM5] | Actinobacillus succinogenes, [GenBank:Asuc_0521], 522 aa, 63 | Neisseria mucosa, [GenBank:EFC88975], 517 aa, 53% |
| 3[GenBank:HSM_0398], 346 aa | [GenBank:HS_1612], 346 aa | [Swiss-Prot BOUWU4/Q01570] | Mannheimia, succiniciproducens [GenBank:MS2260], 346 aa, 78% | Yersinia pseudotuberculosis, [GenBank:YPTB0054], 354 aa, 64% |
| 3[GenBank:HSM_0399], 321 aa | [GenBank:HS_1611], 321 aa | [Swiss-Prot B0UWU5/Q0I571] | Mannheimia succiniciproducens | Providencia rettgeri, [GenBank:EFE51471], [GenBank:MS2259], 321 aa, 67% 322 aa, 63% |
| 4[GenBank:HSM_0856], 314 aa | None | [Swiss-Prot:B0UST9] | None | Rhodopirellula baltica, [GenBank:RB9243], 326 aa, 35% |
| 5[GenBank:HSM_0975a], 157 aa, (CAGT)18 | 5[GenBank:HS_0636], 354 aa, (CAGT)29 | [Swiss-Prot:Q0I2Z7] | Haemophilus influenzae, [GenBank:NTHI0365], 312 aa, 38% | Neisseria meningitidis, [GenBank:AF355193_3], 311 aa, 35% |
| [GenBank:HSM_0975], 231 aa, (AGA)3 | None | [Swiss-Prot:B0UT56] | Pasteurella dagmatis, [GenBank:EEX51329], 334 aa, 40% | Neisseria lactamica, [GenBank:AAN08512], 251aa, 32% |
| 6[GenBank:HSM_0976], 63 aa | [GenBank:HS_0636a] | [Swiss-Prot:B0UT57] | Pasteurella multocida, [GenBank:PM1140], 337 aa, 61% | Streptococcus parasanguinis, [GenBank:ACF35266], 297 aa, 59% |
| 7[GenBank:HSM_0977], 277 aa, (GAGA)11 | None | [Swiss-Prot:B0UT58] | Aggregatibacter actinomycetemcomitans GenBank:D11S_0429], 282 aa, 43% | Neisseria meningitidis, [GenBank:NMC1901], 275 aa, 41% |
| 8[GenBank:HSM_0978], 271 aa, (GA)20 | [GenBank:HS_0637], 158 aa | [Swiss-Prot:B0UT59] | Aggregatibacter actinomycetemcomitans [GenBank:D11S_0429], 282 aa, 43% | Neisseria meningitidis, [GenBank:CAX50883], 280 aa, 39% |
| 9[GenBank:HSM_0979], 293 aa, (CAAT)33 | [GenBank:HS_0638], 297 aa, (CAAT)36 | [Swiss-Prot:B0UT60/Q0I2Z5] | Actinobacillus succinogenes, [GenBank:Asuc_0526], 265 aa, 52% | Photobacterium profundum, [GenBank:PBPRA0217], 253 aa, 41% |
| [GenBank:HSM_1426], 300 aa | None | [Swiss-Prot:B0UUE7] | Haemophilus influenzae, [GenBank:HI0871], 306 aa, 42% | Fusobacterium nucleatum, [GenBank:EDK88896], 326 aa, 38% |
| [GenBank:HSM_1552], 196 aa, (G)9 | [GenBank:HS_1067], 194 aa, (G)6 | [Swiss-Prot:B0UUS9/Q0I479] | None | Edwardsiella ictaluri, [GenBank:NT01EI_2547], 247 aa, 27% |
| 10[GenBank:HSM_1794], 311 aa | None | [Swiss-Prot:B0UWB1] | Mannheimia haemolytica, [GenBank:MHA_0104], 312 aa, 47% | Streptococcus agalactiae [GenBank:AAR29926], 299 aa, 39% |
| [GenBank:HSM_2001], 242 aa, (GGT)3 | [GenBank:HS_0116], 242 aa, (GGT)3 | [Swiss-Prot:B0URS6/Q0I108] | Aggregatibacter aphrophilus, [GenBank:NT05HA_1706], 240 aa, 61% | [GenBank:EER56028], 241 aa, 55% |
| None | [GenBank:HS_0291], 256 aa | [Swiss-Prot:Q0I1K2] | Mannheimia succiniciproducens [GenBank:MS0438], 253 aa, 76% | Vibrio shilonii [GenBank:EDL53925], 259 aa, 62% |
| None | [GenBank:HS_0292], 348 aa | [Swiss-Prot:Q0I1K1] | Actinobacillus minor NM305, [GenBank:EER47697], 340 aa, 57% | Vibrio parahaemolyticus, [GenBank:EED27646], 341 aa, 39% |
1Simple sequence repeats identified within the predicted ORF.
2Identity at the predicted protein level as compared to strain 2336 or strain 129Pt full-length or partial homolog.
3HSM_0398 and 0399 are predicted to encode lipopolysaccharide heptosyltransferases II and I, respectively.
4HSM_0856 contains 28 copies of CAGT 67 bp upstream of the predicted start codon.
5HS_0636 (lob2D), encoding a putative glycosyl transferase [13], is truncated in strain 2336 (HSM_0975a).
6HSM_0976 (lob2C), encoding a putative glycosyltransferase-like protein, has an 117 bp homolog in strain 129Pt (HS_0636a).
7HSM_0977 (lob2B) has been predicted to encode an N-acetylglucosamine transferase [5].
8HSM_0978 (lob2A), encoding a putative glycosyl transferase, had 66% identity to HSM_0977 (lob2B) at the predicted protein level.
9HSM_0979/HS_0638 (lob1) has been predicted to encode a galactosyl transferase [7].
10HSM_1794 contains 4 copies of CAAGCCTACAAGCCTACAAGCCTA 262 bp upstream of the predicted start codon.