Table 1.
List of Antibodies
| Antibody (name and species) |
Source, catalog. & lot no. |
Immunogen | Dilution |
|---|---|---|---|
| Rabbit anti-CGRP | Sigma-Aldrich Inc. Cat. # C8198 Lot # 65K4804 |
Synthetic CGRP (rat) conjugated to KLH (CGRP-KLH). This antibody specifically recognizes the CGRP C-terminal segment (amino acids 24-37) of rat CGRP. |
1:4000 |
| Rabbit anti-ATF-3 | Santa Cruz Biotechnology Cat # sc-188 Lot # F2006 |
The antibody was raised against human ATF-3 (accession No. P18847), the antibody epitope was amino acids 163- 181 in the C-terminus. The antibody was raised against a peptide mapping at the C- terminus of ATF-3 of human origin. Genetic locus: Atf3 (human) mapping to 1q32.3; Atf3 (mouse) mapping to 1 H6. |
1:400 |
| Mouse anti-Neurofilament 200 |
Sigma-Aldrich Inc. Cat. # N0142 Lot # 073K4834 |
The antibody was raised against the carboxyterminal tail segment of enzymatically dephosphorylated pig neurofilament H-subunit. |
1:400 |
| Mouse anti-β (III) Tubulin | Promega, UK Cat # G7121 Lot # 197688 |
Peptide (EAQGPK) corresponding to the C-terminus of β III tubulin. |
1:6000 |
| Rabbit anti-SPRR1A | Prof. Stephen Strittmatter, Yale University Antisera ID. Mocha Bleed #2 |
SPRR1A cDNA (AA230988) was sub- cloned into pTrc-His. Recombinant SPRR1A protein with N terminal His6 tag was purified from transformed E. coli. Rabbits were immunized with SPRR1A- His protein (Bonilla et al., 2002). |
1:7000 (gelatin) 1:20,000 (cryostat) |
| Rabbit anti-PKCγ | Santa Cruz Biotechnology Cat # sc-211 Lot # A0704 |
An oligopeptide corresponding to the carboxyl-terminal end region of 13I PKC (residues 661-671; Ser-Tyr-Thr-Asn-Pro- Glu-Phe-Val-Ile-Asn-Val) was synthesized and used as an immunogen. |
1:200 |
| Rabbit anti-GAP-43 | Dr. G. P. Wilkin, Imperial College London. |
A 610-bp fragment encoding amino acids 17-219 of rat GAP-43 was cloned into the pUR 292 expression vector and expressed in E. coli. The resulting protein was purified and rabbits were inoculated with approx. 100μg of protein in Freund’s complete adjuvant and boosted 1 month later with a further 100 mg in incomplete adjuvant. |
1:2000 |
| Goat anti-IB4 | Vector Labs Ltd., UK Cat # AS-2104 Lot # N0913 |
The antibody was raised in goat against whole lectin 1 of Griffonia simplicifolia. |
1:1000 |