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. 2012 Apr 30;3:158. doi: 10.3389/fmicb.2012.00158

FIGURE 2.

FIGURE 2

Immunization of mice with HF10 followed by lethal HSV-2 challenge via vaginal inoculation. One month after immunization with HF10, 5 × 105 PFUs of wild-type HSV-2 strain 186 were inoculated into mice vagina. Unimmunized mice and UV-inactivated HF10-immunized mice were used as controls. (A) Replication of strain 186 in the genital mucosa was determined by viral titrations of vaginal washes (200 μl). Viral titers were significantly lower in HF10-immunized mice on day 5 than in both unimmunized mice (P < 0.05) and UV-inactivated HF10-immunized mice (P < 0.05). (B) Clinical symptoms were monitored and scored for 8 days. HF10-immunized mice had lower disease scores than unimmunized mice (on day 8, P < 0.0001). Disease scores were not significantly different between UV-inactivated HF10-immunized mice and unimmunized mice (P = 0.065). (C) Survival curve derived by the Kaplan–Meier method. HF10-immunized mice survived longer than unimmunized mice and UV-inactivated HF10 mice (on day 28, P < 0.0001 and P = 0.001, respectively).