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. 2012 Jan 6;120(4):541–546. doi: 10.1289/ehp.1104051

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Detection of Ki67 expression in MCF‑7 and MCF‑7/ADR cells (A) and in OVCAR‑3, HeLa, and CHO cells (B) by immunofluorescence. Cells were grown, starved overnight without serum, treated with different doses of PBDE‑209 for 72 hr in medium containing 1% serum, and then subjected to immunofluorescence analysis of Ki67 expression. Cells treated with 0.1% DMSO were used as vehicle control (VC). The experiments were performed three times. Ki67 positivity is presented as the ratio of Ki67-positive cells to the number of all cells in that cell line; data shown are mean ± SD. *p < 0.05 versus VC. **p < 0.01 versus VC. ^#p < 0.05 versus MCF‑7/ADR cells (A) or versus CHO cells (B) in the same treatment group.