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. 2012 Jan 19;120(4):508–514. doi: 10.1289/ehp.1104282

Figure 2.

Figure 2

Regulation of cytokines involved in obesity-related inflammation. mRNA levels were assessed by qRT-PCR of commonly regulated pro- and anti-inflammatory cytokines in obesity-related low-grade inflammation in undifferentiated cells (A) and adipocytes (B) treated with a single concentration of TCDD (25 nM), PCB-126 (1 μM), or PCB-153 (10 μM). Gene expression was normalized by HPRT or TBP expression using the ΔΔCt method and is expressed as mean of fold induction ± SE. (C) Measurement of proinflammatory cytokine secretion by Luminex assay in the supernatants of undifferentiated cells or adipocytes treated with 25 nM TCDD or 1 μM PCB-126 for 48 hr. Data are expressed as mean ± SE. *p < 0.05, compared with control by Mann–Whitney U-test.