Table 1.
Forward and reverse primer sequences, repeat motifs and expected sizes of microsatellite loci and its status of amplification of SSR loci of date palm
| Primer name | Primer’s sequence | Tm (°) | Expected size | Motif repeat | Status of amplification | |
|---|---|---|---|---|---|---|
| DP150 | F | CTGCGCCAATCTAAACCATT | 52.6 | 177 | (GAA)9 | − |
| R | GCAAATTGCAACAAATCCTTG | |||||
| DP151 | F | TTGCTGGTTGAAATGGTGTT | 53.3 | 168–186 | (AC)37 | ++ |
| R | GCAACAGATGCTCTTGCTCA | |||||
| DP152 | F | ACGAGTTTTTGGGAGAGCAA | 53.6 | 224 | (TAT)8 | − |
| R | GCAAGTTGCCAACATTCTTGT | |||||
| DP153 | F | TCATCACAGGCAATGGCTAA | 51.6 | 204 | (TCA)9 | + |
| R | GCAGATGGCCATTGAACC | |||||
| DP154 | F | ACACACACACACCGCGAAT | 53.1 | 249 | (AC)19 | + |
| R | GCATGTGAGGCGCATATCTA | |||||
| DP155 | F | CCCCCTCTCTCTCTCTCTCTC | 55.8 | 200 | (TC)51 | − |
| R | GCCAAGAGGATTGGAGATTG | |||||
| DP156 | F | TGTGTGTGTGTGTGTGTGTGA | 54.6 | 221 | (GA)17 | + |
| R | GCCATTGTTTGTGTGGACTG | |||||
| DP157 | F | TGGACAATGACACCCCTTTT | 54.6 | 180–244 | (TC)19 | ++ |
| R | GCCCACACAACAACCTCTCT | |||||
| DP158 | F | TCATTGGCTAATCCACACACA | 54.3 | 204 | (GA)29 | − |
| R | GCCTTGTGGTCATGAGAGGT | |||||
| DP159 | F | AGCTCCAATTTGCTGCAGAG | 54.3 | 156–172 | (TC)27 | ++ |
| R | GCTGACCTGGAGTCCAAAAC | |||||
| DP160 | F | AAGAGCGACAATCATGACCA | 57.7 | 108–136 | (GAAA)5 | ++ |
| R | GGAAATTGAAGGGCATCTTG | |||||
| DP161 | F | TGGTTGCTGCTTATCTGCTG | 54.9 | 211 | (CT)13 | + |
| R | GGAGGGAACCGAGAGAGAGA | |||||
| DP162 | F | TGGACTTCAAGAAGTGCGAAT | 57.3 | 183 | (TACA)9 | − |
| R | GGCAGTCACATTTTGCTTCA | |||||
| DP163 | F | GTGCGTGTGTGTGTGTGTGT | 53.4 | 215 | (GA)19 | + |
| R | GGCTGTTTGGGTTCGTACTG | |||||
| DP164 | F | GGACCAAGAACCGACAGTTG | 57.1 | 200 | (ATAG)6 | + |
| R | GGGAAGGTGAGGTGGTATGA | |||||
| DP165 | F | AAGCATCCTATGGCTTTGACA | 54.8 | 222 | (AATA)5 | + |
| R | GGGCTGTATGTGATGCATTG | |||||
| DP166 | F | CAATTTCTTCTCGCCTGGAG | 54.5 | 210 | (GAAA)5 | + |
| R | GGGGTTTCTTTTCCTTCTGC | |||||
| DP167 | F | ACATCCAATGGCATCCAAAT | 57.6 | 243 | (GAAA)6 | + |
| R | GGGTTTCCAGGTTTTCTTCTC | |||||
| DP168 | F | GCAGCAAAGCCCTTAGGC | 54.3 | 163–175 | (CAT)8 | ++ |
| R | GGTGTTATGTGCAGCCAATG | |||||
| DP169 | F | GCATGGACTTAATGCTGGGTA | 57.1 | 129–223 | (AAT)12 | ++ |
| R | GGTTTTCCTGCCAACAACAT | |||||
| DP170 | F | TCTTTGGGCTTACGACAACC | 55.9 | 195–227 | (AGGG)5 | ++ |
| R | GTATGGCCCAAGATGCAGAT | |||||
| DP171 | F | GTGGGAGTAGCGAGGTATGG | 56 | 197–218 | (TTC)10 | ++ |
| R | GTCCGGCACTTTAGGAAGTT | |||||
| DP172 | F | GGTGTTTGGGCCTATTTCCT | 54.2 | 199–235 | (AGG)11 | ++ |
| R | GTCCTCCTCCTCCTCTGTCC | |||||
| DP173 | F | CCACATGCAATTTCCAAAAG | 51.9 | 198 | (TC)27 | + |
| R | GTGCGTATCGGGAGAGAGAG | |||||
| DP174 | F | CTCTGTCGTACGGAGGAAGG | 55.6 | 187 | (CGTG)5 | − |
| R | GTGGCACTATCACGCTCTCA | |||||
| DP175 | F | ACACACACACACACACACACC | 57.6 | 196–274 | (CA)19 | ++ |
| R | GTGGCTTCTTTTTGGCTGTC | |||||
| DP176 | F | GCCATTAACGAAATGGCTTG | 54.5 | 198 | (CAA)9 | + |
| R | GTTTGCACATAGCGCTTCAA | |||||
| DP177 | F | TTCCTTGGGCTCACTTCAAC | 54 | 216 | (AGGC)6 | + |
| R | TAACATGCCAGCAAAGGTGA | |||||
| DP178 | F | AGTTTGTCAGGCCATTTGGT | 58.7 | 186 | (TC)19 | + |
| R | TACATGTGCGTATCGGGAGA | |||||
| DP179 | F | GGTTAGCCATCCAAAAGTGG | 56.2 | 183 | (ATTT)5 | − |
| R | TATGTAGCCTCCACCGCATC |
−, Non-amplification; +, amplification of monomorphic band; ++, amplification of polymorphic band of desired PCR product with Qatari date palm