Table 1.
Results of sulfate reduction with different electron acceptors in enrichment cultures
| Growth substrate (concentration) | Sulfate reduction |
|---|---|
| Extracts (0.2%): | |
| Tryptone | ++ |
| Peptone | ++ |
| Alcohols (0.2%): | |
| Ethanol | – |
| Glycerol | ++ |
| Organic acids (0.2%): | |
| Acetate | – |
| Propionate | – |
| Formate | + |
| Pyruvate | ++ |
| Malonate (5 mM) | – |
| Adipate (5 mM) | + |
| Aromatic compounds (4 mM): | |
| Benzoate | – |
| 3-Hydroxybenzoate | – |
| 4-Hydroxybenzoate | – |
| 2,5-Dihydroxybenzoate | – |
| 3-Chlorobenzoate (2 mM) | – |
| Phenylacetate | – |
| 4-Hydroxyphenylacetate | – |
| Cinnamate | – |
| 4-Hydroxycinnamate | – |
| 2-Hydroxycinnamate | – |
| 2-Methoxybenzoate | +a |
| 4-Methoxybenzoate | +a |
| Vanilline | – |
| Syringate | – |
| Control (no substrate) | – |
A deep-well U-bottom microtiter plate was prepared with various substrates listed below and sulfate reduction determined using the methods described in the text
Sulfate reduction is scored as: ++ strong, + weak, − no sulfate reduction
aSubsequent subculture of the enrichment failed to grow