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. 2011 Dec 8;18(5):265–279. doi: 10.1093/molehr/gar078

Figure 2.

Figure 2

Phosphorylation of MYL and MYPT1 is reduced in isolated myometrial strips treated with ROCK inhibitor g-H-1152. Myometrial strips treated with 10 nM OXT with and without cumulative additions of g-H-1152 to a maximum of 10 μM (as in Fig. 1), were snap frozen at the end of the experiment during a relaxed phase. (A) Solubilized proteins were subject to SDS–PAGE and immunoblotting for ppMYL (Thr18/Ser19), pMYL (Ser19), MYL, pMYPT1 (Thr853), pMYPT1 (Thr696) and MYPT1.(B–E) Signal from the phospho-specific antibody was normalized to that obtained from the associated total antibody, and expressed as a percentage of the OXT-treated value. Bars represent mean + SEM, and statistical analyses were performed using paired Student's t-tests, *P < 0.05, **P < 0.01, ***P < 0.001, n = 5.