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. 2011 Dec 8;18(5):265–279. doi: 10.1093/molehr/gar078

Figure 4.

Figure 4

Spontaneous phasic contractions of freshly isolated myometrium are associated with ROCK-dependent increases in phosphorylation of MYL (Thr18/Ser19) and MYPT1 (Thr853). Spontaneously contracting myometrial strips were treated with or without 1 μM g-H-1152 for 40 min (A and B). Strips were subsequently snap-frozen at the peak of (contracting), or immediately following (relaxed), a phasic contraction. Solubilized proteins were subjected to SDS–PAGE and immunoblotting. Signals from phospho-specific antibodies were normalized to those obtained from their associated total antibodies, and data were expressed as a percentage of the control value (OXT, relaxed). (C) ppMYL (Thr18/Ser19) levels, (D) pMYL (Ser19) levels, (E) pMYPT1 (Thr853) levels, (F) pMYPT1 (Thr696) levels; bars represent mean + SEM, n = 7. Data were analysed using two-way ANOVA (‡P < 0.05 for the overall effect of g-H-1152) and Bonferroni post hoc tests to determine both the contribution of contraction (*P < 0.05 and **P < 0.01) and of g-H-1152 treatment on contracting tissue (††P < 0.01).