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. 2011 Dec 8;18(5):265–279. doi: 10.1093/molehr/gar078

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© The Author 2011. Published by Oxford University Press on behalf of European Society of Human Reproduction and Embryology

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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ROCK inhibitor g-H-1152 causes dose-dependent decreases in MYL and MYPT1 phosphorylation in cultured myometrial cells. Resting myometrial cells were treated for 40 min with g-H-1152 at the concentrations shown; 0.1% DMSO was added to control wells. (A) Cell lysates were subjected to immunoblotting for total and phosphorylated MYL and MYPT1, representative blots are shown. Signal from the phospho-specific antibody was normalized to that obtained from the associated total antibody, and was expressed as a percentage of the non-treated value. (B) ppMYL (Thr18/Ser19) levels; (C) pMYL (Ser19) levels; (D) pMYPT1 (Thr853) levels and (E) pMYPT1 (Thr696) levels. Data points represent mean ± SEM, n = 11. Statistical significance was determined using one-way ANOVA and Dunnett's post hoc test; *P < 0.05 and **P < 0.01 compared with non-treated cells.