Fig. 1.

Prolonged exposure to insulin increases mitochondrial content of cholesterol in hepatocytes. Hepa1c1c7 cells were incubated with insulin for 16 h (n = 3). Mitochondria were then isolated and mitochondrial content of free cholesterol was quantified (A). Levels of phosphorylated (P-Akt), total Akt (T-Akt), and β-actin in these same cells were evaluated by immunoblotting with specific antibodies (B). Results represent mean ± se of three independent experiments. **, P < 0.01 vs. no insulin.