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Prolonged exposure to insulin increases mitochondrial content of cholesterol in liver of mice. B6 mice were treated with the long- and slow-acting insulin (glargine) for 8 wk as detailed in Materials and Methods. Liver mitochondria were immediately isolated after animals were killed. Mitochondrial content of cholesterol was quantified. Results represent mean ± sd of six mice per group. **:, P < 0.01.
