Fig. 4.

Exposure of isolated mitochondria to cholesterol increases ROS production. Mitochondria isolated from primary hepatocytes were incubated with cholesterol bound to BSA at noted concentration for 5 min and then with carboxy-H2-DCF-DA for 30 min. ROS levels were subsequently quantified as detailed in Materials and Methods. Results represent mean ± se of three independent experiments. **, P < 0.01 vs. no cholesterol.