Table 1.
Multiplex cytokine analysis of human CTCL cell lines stimulated with TLR ligands*.
| V-CAM-1 | RANTES | ICAM-1 | IP10 | VEGF | PAI-1 | IL-10 | sFas-L | IL-13 | LIF | TRAIL | Resistin | IL-lα | GM-CSF | MIP1α | TNFβ | |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| HH, unstimulated | 6603 | 1213 | 737 | 189 | 199 | 6 | 1 | 23 | 0 | 0 | 15 | 1 | 8 | 10 | 26 | 0 |
| HH, ODN2216 | 4541 | 772 | 598 | 88 | 180 | 5 | 1 | 15 | 0 | 0 | 13 | 1 | 6 | 6 | 19 | 0 |
| HH, ODN2336 | 3570 | 838 | 462 | 135 | 186 | 4 | 0 | 15 | 0 | 0 | 11 | 1 | 8 | 4 | 10 | 0 |
| HH, ODN2006 | 4762 | 467 | 624 | 341 | 189 | 6 | 1 | 18 | 1 | 0 | 16 | 1 | 19 | 7 | 45 | 0 |
| HH, imiquimod | 4281 | 807 | 419 | 125 | 139 | 4 | 1 | 18 | 0 | 0 | 10 | 1 | 7 | 9 | 7 | 0 |
| HuT78, unstimulated | >70 294 | 866 | 426 | 253 | 218 | 168 | 703 | 267 | 84 | 47 | 29 | 50 | 13 | 29 | 12 | 25 |
| HuT78, ODN 2216 | >70 294 | 409 | 222 | 298 | 193 | 142 | 620 | 199 | 62 | 44 | 23 | 36 | 13 | 19 | 3 | 29 |
| HuT78, ODN 2336 | >70 294 | 581 | 242 | 381 | 284 | 141 | 780 | 247 | 66 | 48 | 27 | 51 | 18 | 23 | 10 | 32 |
| HuT78, ODN 2006 | >70 294 | 500 | 267 | 612 | 214 | 1175 | 283 | 110 | 68 | 51 | 23 | 13 | 26 | 79 | 9 | 21 |
| HuT78, imiquimod | >70 294 | 672 | 259 | 270 | 158 | 115 | 638 | 178 | 92 | 44 | 22 | 45 | 13 | 26 | 3 | 33 |
| PBMC, unstimulated | 1 | 3 | 79 | 23 | 3 | 148 | 1 | 8 | 0 | 0 | 10 | 36 | 1 | 0 | 5 | 1 |
| PBMC, ODN 2216 | 11 | 4 | 106 | 3372 | 3 | 216 | 7 | 19 | 0 | 2 | 56 | 37 | 46 | 1 | 17 | 6 |
| PBMC, ODN 2336 | 14 | 5 | 100 | >5000 | 3 | 211 | 10 | 22 | 0 | 3 | 36 | 31 | 50 | 1 | 19 | 7 |
| PBMC, ODN 2006 | 12 | 43 | 249 | 84 | 8 | 1258 | 50 | 10 | 0 | 4 | 101 | 63 | 6 | 2 | 179 | 36 |
| PBMC, imiquimod | 11 | 4 | 174 | 5 | 27 | 293 | 20 | 8 | 2 | 2 | 187 | 83 | 10 | 4 | 54 | 2 |
CTCL, cutaneous T cell lymphoma; PBMC, peripheral blood mononuclear cell; sVCAM-1, soluble vascular cell adhesion molecule 1; RAN TES, regulated upon activation, normal T cell expressed and secreted; sICAM-1, soluble intercellular adhesion molecule 1; IP10, interferon inducible protein 10; VEGF, vascular endothelial growth factor; PAI-1, plasminogen activator inhibitor 1; IL-10, interleukin 10; sFas-L, soluble Fas ligand; LIF, leukemia inhibitory factor; TRAIL, TNF-related apoptosis-inducing ligand; GM-CSF, granulocyte-macrophage colony stimulating factor; MIP1α, macrophage imflammatory protein 1α; TNFβ, tumor necrosis factor β
Cells were stimulated with Toll-like receptor (TLR) ligands as shown. After 72 h, supernatants were harvested and analyzed for the presence of 51 soluble factors using a bead-based cytokine assay. Only data for soluble factors scoring as detectable (>15 pg/mL above background) for HH and/or HuT78 cells are shown. Note that HuT78 values for VCAM-1 were elevated beyond the scale of the reference standard curve.