Table 4.
Cytokine and chemokine-related genes, and oxidative stress inhibitors differentially or predominantly expressed in DA.ACI(Cia10) or DA.
| Gene symbol | Gene name | Accession number | Expressed in all samples
|
Preferential strain expression
|
|||
|---|---|---|---|---|---|---|---|
| Fold-difference | P-value** | DA positive (%) | Cia10 positive (%) | P-value** | |||
| Genes up-regulated in DA | |||||||
| Ccl21b§ | chemokine (C-C motif) ligand 21b | NM_001008513.1 | 1.61 | 0.002 | |||
| Csk | c-src tyrosine kinase | XM_236290.3 | 2.02 | 0.002 | |||
| IL-11 | interleukin-11 | XM_346531.2 | 1.66 | 0.007 | |||
| IL-3ra | interleukin-3 receptor subunit alpha | NM_139260.1 | 1.52 | 0.009 | |||
| Genes predominantly expressed in DA | |||||||
| Ccl12 | chemokine (C-C motif) ligand 12 | XM_213425.2 | 11 (92) | 2 (33) | 0.021 | ||
| Ccr6§ | chemokine (C-C motif) receptor | NM_001013145.1 | 12 (100) | 3 (50) | 0.024 | ||
| Cx3cr1 | chemokine (C-X3-C motif) receptor 1 | NM_133534.1 | 10 (83) | 1 (16) | 0.012 | ||
| Cxcl10 | chemokine (C-X-C motif) ligand 10 | NM_139089.1 | 12 (100) | 3 (50) | 0.024 | ||
| Cxcr3 | chemokine (C-X-C motif) receptor 3 | NM_053415.1 | 12 (100) | 3 (50) | 0.024 | ||
| Ifi47 | interferon gamma inducible protein 47 | NM_172019.1 | 9 (75) | 0 (0) | 0.009 | ||
| IL-10 | interleukin 10 | NM_012854.1 | 8 (67) | 0 (0) | 0.012 | ||
| IL-1rl1 (IL-33R) | interleukin 1 receptor-like 1 | NM_013037.1 | 12 (100) | 2 (33) | 0.005 | ||
| IL-6 | interleukin-6 | NM_012589.1 | 11 (92) | 2 (33) | 0.021 | ||
| IL-17A | interleukin-17A | NM_001106897 | 13 (93) | 3 (43) | 0.025 | ||
| Stat4§ | signal transducer and activator of transcription 4 | NM_001012226.1 | 11 (92) | 2 (33) | 0.021 | ||
| Genes up-regulated in DA.ACI(Cia10) | |||||||
| Ccl11 | chemokine (C-C motif) ligand 11 (eotaxin) | NM_019205.1 | 1.93 | 0.003 | |||
| Tgfa | transforming growth factor alpha | NM_012671.1 | 4.20 | 0.006 | |||
| Tgfb2 | transforming growth factor beta 2 | NM_031131.1 | 1.93 | 0.0001 | |||
| Tnfsf12 | tumor necrosis factor ligand superfamily member 12 (TWEAK) | NM_001001513.2 | 2.21 | 0.003 | |||
| Cat* | catalase | NM_012520.1 | 2.07 | 0.008 | |||
| Gstm1* # | glutathione S-transferase mu 1 | NM_017014.1 | 2.71 | 0.003 | |||
| Mt3* | Metallothionein-3; growth inhibitory factor (GIF) | NM_053968.2 | 3.34 | 0.009 | |||
| Nfe2l1* | nuclear factor, erythroid derived 2,-like | XM_340886.3 | 1.76 | 0.009 | |||
| Sqstm1* | sequestosome 1 | NM_175843.3 | 1.66 | 0.002 | |||
| Genes predominantly expressed in DA.ACI(Cia10) | |||||||
| Tgfbr3 | transforming growth factor, beta receptor III | NM_017256.1 | 6 (50) | 6 (100) | 0.053 | ||
| Vdr | vitamin D (1,25- dihydroxyvitamin D3) receptor | NM_017058.1 | 4 (33) | 4 (80) | 0.321 | ||
§
genes associated with genetic susceptibility in rheumatoid arthritis;
#
gene associated with disease severity in rheumatoid arthritis. Grey box: gene implicated in Th17 cell responses.
*
Inhibitors of oxidative stress;
**
Genes expressed in all samples were compared with t-test, and preferecial strain expression with Fisher Exact Test.
IL-17A results are shown in BOLD and reflect qPCR data of 14 DA and 7 DA.ACI(Cia10) rats.