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. Author manuscript; available in PMC: 2012 Apr 30.
Published in final edited form as: Science. 2009 Jan 9;323(5911):256–259. doi: 10.1126/science.1166226

Fig. 2.

Fig. 2

HDAC4 is required for retinal cell survival and rescues BP cells from naturally occurring cell death. RNAi vectors targeting GAPDH or HDAC4 were electroporated into the wild type mouse retina at P0, with assay at P6 (A–C). (A) Cryosections from retinas with GAPDH RNAi, (B) HDAC4 RNAi, (C) HDAC4 shRNA and an RNAi resistant HDAC4-expressing construct. (D) Clonal analysis at P21 using replication-incompetent retrovirus LIA, or LIA-HDAC4, injected at P0. LIA-HDAC4 clones exhibited an increase in the percentage of BP cell-containing clones, and a reduction in the percentage of 1 rod clones. 800–1000 clones were analyzed on each of 3 retinas infected by LIA or LIA-HDAC4. *** p<0.05 by Student’s t-Test. (E) Retinal section showing a typical 1 rod clone that comprises the majority of the clone types. (F) Retinal section showing a typical 1 rod plus 1 BP clone that was the most increased clone type labeled by LIA-HDAC4.