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. Author manuscript; available in PMC: 2012 Apr 30.
Published in final edited form as: Nat Neurosci. 2008 Dec 7;12(1):44–52. doi: 10.1038/nn.2234

Figure 7.

Figure 7

Increased levels of LAMP-2 at the lysosomal membrane. (a–c) Immunofluorescence on retinal flat mounts where LAMP-2 is shown in green, red/green opsin in red and blue signal shows nuclear DAPI stain. Insets in upper right corner (with box) show enlarged cells (arrow). (a) Wild type retinae at PW5 showing lysosome (small green dots) with normal LAMP-2 distribution. Weak red/green opsin signal is detected at the level of the PR nuclei since in wild type it is mainly found in the OSs. (b, c) PDE-β mutant at PW5. (b) Enlarged lysosomes (dots) due to accumulation of LAMP-2 at the lysosomal membrane are seen specifically in cones. (c) Confocal section of same field as in (b) taken at the level of the inner nuclear layer showing levels of LAMP-2 similar to those in wild type (a). (d) qRT-PCR for the 3 different LAMP-2 splice forms showing the relative concentration and the ratios between the PDE-β mutant and wild type.