Fig. 1.

Radiation sensitivity of Drosophila larvae in the presence of Minute mutations or Bouvardin. Survival after each treatment is expressed as the fraction of animals that emerged (‘eclosed’) as viable adults. The averages have been normalized to the average fraction eclosed in no IR or drug control for the respective genotype. Error bars extend beyond fractional survival of 1.0 simply because of normalization. (A–C) Fraction eclosed for wild type (WT) and grp and p53 mutants at 16 μM of Bouvardin, with and without IR. The data are averages of three to eight biological replicates per sample. Dotted line on each graph denotes expected ‘fraction eclosed’ if drug and IR act additively. IR doses were 4000 rad for WT and grp, and 3000 rad for p53 because of the higher radiation sensitivity of the latter. (D) Fraction eclosed for the Minute mutant and yw controls after exposure to 0 (–IR) and 2000 (+IR) rad of X-rays. n ranged from 86 to 207 per genotype per treatment, in three biological replicates per sample. *P<0.01; **P<0.001. 100 rad=1 Gy. Error bar=1 s.d.