Fig. 5.

IL-1β-induced repression of smooth muscle myosin heavy chain (SMMHC) and smooth muscle (SM) α-actin protein levels are NF-κB dependent. A: representative images of Western blot analysis of multiple smooth muscle cell marker genes from cell lysates taken after 72 h of treatment with IL-1β (IL, 5 ng/ml) or vehicle (V) in cells infected with empty adenovirus (AdE) or IκBαSR-expressing adenovirus (AdIκB). GAPDH represents the loading control. Arrow, SM1 isoform of SMMHC (lower band is nonmuscle MHC). Densitometric quantitation of band intensity for SM α-actin (B), calponin-1 (C), SMMHC (D), and SM22α (E) normalized to GAPDH levels. All values were then normalized to the Ad-Empty vehicle control group. Data represent means ± SE from 3 independent experiments.