Table 1. Primer Sequences for Real Time PCR.

Gene	Sequence ID	Forward primer	Reverse primer
GAPDH	NM_002046.3	TGCACCACCAACTGCTTAGC	GGCATGGACTGTGGTCATGAG
CLDN 2	NM_016675.4	ATGGCCTCTCTTGGCCTCCAA	TCACACATACCCTGTCAGGCT
CLDN 4	NM_001305.3	ACAGACAAGCCTTACTCC	GGAAGAACAAAGCAGAG
MLCK	NM_053025.3	GCCTGACCACGAATATAA	GCTCCTTCTCATCATCATC
OCLN	NM_002538.2	TCAGGGAATATCCACCTATCACTTCAG	CATCAGCAGCAGCCATGTACTCTTCAC
ZO-1/TJP-1	NM_003257.3	AGGGGCAGTGGTGGTTTTCTGTTCTTTC	GCAGAGGTCAAAGTTCAAGGCTCAAGAGG

GADPH: glyceraldehyde-3-phosphate dehydrogenase; CLDN 2: claudin 2; CLDN 4: claudin 4; MLCK: myosin light chain kinase; OCLN: occludin; ZO-1: zonula occludens 1/tight junction protein 1.