Fig. 3.
Dual-voltage clamp in patterned cell pairs and analysis of single gap junction channels. A: illustration of four measurements showing openings of single gap junction channels to a full open state and to substates. Note that, depending on polarity of junctional voltage (Vj), the channels have a different conductance. This suggests the presence of mixed Cx43/Cx45 channels (see discussion). B: histogram summarizing the measurements of single channels. The Gaussian fit shows a peak at 45 pS (±0.8 pS; R2 = 0.96; n = 745).