Fig. 2.

Tracheal mucus obstruction and increased bronchoalveolar lavage (BAL) mucus content are features of congenic Scnn1b-Tg mouse strains. A: correlation between the percentage of 3-day-old Scnn1b-Tg pups with visible tracheal mucus plugs at dissection and survival. n = same as in Fig. 1B. B: progressive incidence of tracheal mucus plugging in C3H/HeN mice. n = number of mice/time point. Control WT mice (n = 6 or 7 per time point) showed no evidence of tracheal mucus plugging (not shown). C: Agarose gel Western blots. D: corresponding densitometry of unfractionated BAL samples from 5-day-old Scnn1b-Tg mice and WT littermates from the various strains. Membranes were probed with a murine Muc5b antibody. n, Number of mice/group; a.u., arbitrary units. E: correlation between the fold increase in Muc5b BAL content in Scnn1b-Tg compared with WT littermates and survival at 10 days (from Fig. 1A). n, same as D. F: total protein content of BAL fluid from the samples studied in Fig. 2. C–E: *P < 0.05 vs. WT littermates. n, number of mice/group. For all panels, *P < 0.05 vs. WT littermates, #P < 0.05 vs. C57BL/6N of the same genotype.