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. 2012 Apr 26;444(Pt 1):89–96. doi: 10.1042/BJ20120209

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© 2012 The Author(s) The author(s) has paid for this article to be freely available under the terms of the Creative Commons Attribution Non-Commercial Licence (http://creativecommons.org/licenses/by-nc/2.5/) which permits unrestricted non-commercial use, distribution and reproduction in any medium, provided the original work is properly cited.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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(A) Coro1A, Coro1B, Coro1C–R28D, Coro1C–2×KE, Coro1C–R28D/2×KE (0.1 μM) or Coro1C (0.3 μM) was incubated with increasing concentrations of actin and cosedimentation assays were performed. Supernatant fractions were separated by SDS/PAGE and analysed by immunoblotting with an anti-His antibody. Representative immunoblots are from at least four independent experiments. Quantification of the amount of Coro1A (B), Coro1B (C), Coro1C (D), Coro1C–R28D (E) and Coro1C–2×KE (F) bound to F-actin as a function of the free F-actin concentration. Each data point is shown as mean±S.E.M. from at least four independent experiments. Binding curves were fitted to the experimental data and used to generate a Hill coefficient (h) and dissociation constant (K_d), which are displayed on the plots.