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Effect of 48 h treatment with M22 (50 or 100 ng/ml), IGF-I (10 ng/ml), the specific IGF-IRα blocking antibody 1H7 (5 μg/ml), or either M22 or IGF-I in combination with 1H7 on HA levels in GO orbital fibroblast cultures (n = 6). ELISA results are expressed as mean ± sem fold change in HA level relative to parallel untreated cultures (medium alone) or to parallel control cultures treated with M22 or IGF-I alone. *, P < 0.05.
