Skip to main content
. 2012 Mar 9;53(3):1234–1243. doi: 10.1167/iovs.11-8834

Figure 4.

Figure 4.

Western blot analysis of peptide-N-glycosidase F (N-Glycanase)-treated total protein extracted from chick embryonic, hatchling, and adult corneas. NCAM isoforms were detected using the anti-NCAM extracellular domain antibody (A) and anti-NCAM cytoplasmic domain antibody (B). Four NCAM isoforms, NCAM-180, -120, -96, and -87 with apparent MWs of 160 kDa, 110 kDa, 96 kDa, and 87 kDa, respectively, were found in E7 chick cornea. NCAM-180, -120, and another isoform, -92, with an apparent MW of 92 kDa were detected in later developmental stages. NCAM-120 and -92 were detected in 20 weeks adult corneas. Only one isoform which was identified as NCAM-180 showed immunoreactivity to the antibody against the intracellular domain (B). The intensity of the bands on each lane in (A) was profiled using commercial software (ImageQuant TL; GE Health Care Biosciences Corp.) (C). The relative amount of each isoform of NCAM at the selected stages was determined (ImageQuant TL; GE Health Care Biosciences Corp.) (D). The insets in (C) are zoom-in profiles of NCAM isoforms in E7 and 20 weeks adult corneas.