Figure 3.

Upregulation of MMP-1 and MMP-3 transcripts and proteins by TNF-α or IL-1β in normal and CCh fibroblasts. In the same cultures as described in Figure 2, expression of MMP-1 and MMP-3 transcripts in normal fibroblasts (□) and CCh fibroblasts (■) was detected by qRT-PCR (A, #P < 0.01 vs. normal; *P < 0.01 vs. control). Both proMMP-1 and actMMP-1 (top) and proMMP-3 and actMMP-3proteins (bottom) in cell lysates and culture media were monitored by Western blot analysis (B).