Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Knockdown efficiency of TSG-6 siRNA. Both normal and CCh fibroblasts were cultured in DMEM with 0.5% FBS for 48 hours before transfection with 100 nM TSG-6 siRNA or scRNA for another 48 hours. qRT-PCR showed that the TSG-6 transcript was markedly downregulated using GAPDH as the internal control (A, *P < 0.01 vs. scRNA). Western blot analysis also showed that the TSG-6 protein was notably downregulated using β-actin as the loading control (B).
