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Upregulation of MMP-1 and MMP-3 transcripts in normal and CCh fibroblasts by TSG-6 knockdown. Both normal and CCh fibroblasts were cultured in DMEM with 0.5% FBS for 48 hours and were transfected with TSG-6 scRNA (□) or siRNA (■) for another 48 hours without (A) or with (B) IL-1β added for the last 24 hours. Cell lysates were collected for MMP-1 and MMP-3 mRNA detection by qRT-PCR. *P < 0.01 versus scRNA.
