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Upregulation of MMP-1 and MMP-3 protein and actMMP-1 by TSG-6 knockdown. In the same cultures described in Figure 4, cell lysates were collected for Western blot analysis of proMMP1and actMMP-1 proteins (top) and MMP-3 proteins (bottom) in cell lysates and culture media of both normal and CCh fibroblasts without (A) and with (B) stimulation of IL-1β using β-actin as the loading control.
