Table 2.

TSA Treatment of DBA/2J^R3 Mice Does Not Affect Development of Ocular Hypertension

Treatment	n (Eyes)	Mean IOP (mm Hg ±SD)*	IOP Range (mm Hg)	Mean AC Score (± SD)†	Buphthalmia‡
DMSO	46	20.56 ± 7.82	9–40	1.2 ± 0.7	26.1%
TSA	50	19.88 ± 5.96	7–35	1.6 ± 0.9	42.0%
No injection	48	20.13 ± 6.43	11–41	1.6 ± 0.7	22.9%

^*Mean intraocular pressure (IOP) in 6-month-old mice is 12.5 ± 3.0 mm Hg (range, 8–20, n = 18). Note that IOPs were taken under ketamine and xylazine anesthesia, which may yield artificially low values, particularly in ocular hypertensive mice.¹³ There was no statistical difference between IOP levels of all treated groups (ANOVA, P = 0.307), but all treated groups were significantly different from 6-month-old mice (P << 0.001). These values are also comparable to the mean IOPs reported for age-matched DBA/2J animals that do not carry the R3 allele.¹⁷

^†Anterior chamber (AC) score was based on a qualitative evaluation of iris pigment dispersion and transillumination defects by two observers. Scores were made on a 1 to 4 scale, where 1 is no damage and 4 is severely damaged, and the average taken. All treated groups exhibited significantly more AC defects than 6-month-old mice (P < 0.003), but there was no significant difference among treated groups (P > 0.32).

^‡Buphthalmia, which is characterized by bulging of the eye from the orbit as a consequence of elevated IOP, scored as + or − based on agreement between two observers. The value given is the percentage of eyes in the cohort that were scored as “+.”