Figure 2.

Involvement of PI 3-kinase in IL-1β–induced IκB degradation pathways. When cells reached approximately 70% confluence, they were starved of serum for 24 hours. The serum-starved cells were pretreated with LY294002 for 2 hours and then treated with IL-1β for 10 minutes. The IL-1β–treated cells were maintained in DMEM for the designated time. Total cell protein preparation and immunoblot analysis were performed as previously described in Figure 1. β-actin was used to control protein concentration on immunoblot analysis. The results represent data obtained in three independent experiments.