Figure 5.

Inductive activity of IL-1β on FGF-2 production in hRPE cells. When hRPE cells reached approximately 70% confluence in each culture passage, they were starved of serum for 24 hours. The serum-starved cells were treated with IL-1β (5 ng/mL) for 10 minutes and then maintained in DMEM for 16 hours. The cell lysate preparation and immunoblot analysis were performed as described in Figure 1 with the anti–FGF-2 antibody. β-actin was used to control protein concentration on immunoblot analysis. 1st, 2nd, and 3rd, designate the first, second, and third passage cells. The results represent data obtained in three independent experiments.