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Induction of adipocyte differentiation from TMSCs. TMSCs were cultured in ADM and AMM alternately for adipogenic induction. Oil red O (red) staining was compared on TMSCs in SCGM (A) and in ADM/AMM (B). Hematoxylin stains nuclei blue. Bars: 50 μm. (C) Adipocytic markers ap2 and leptin from TMSCs in SCGM and induced cells in ADM/AMM were quantified by qRT-PCR. Error bars show SD of triplicate analyses. *P < 0.05 (n = 3, Student's t-test). ap2, adipocyte protein 2.
