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. 2012 Mar 21;53(3):1566–1575. doi: 10.1167/iovs.11-9134

Table 2.

Primer Sequences Used in Quantitative RT-PCR

Gene Name DNA Sequence
18S Ribosomal RNA Forward: CCCTGTAATTGGAATGAGTCCAC
Reverse: GCTGGAATTACCGCGGCT
ABCG2 Forward: TGCAACATGTACTGGCGAAGA
Reverse: TCTTCCACAAGCCCCAGG
Pax6 Forward: CAATCAAAACGTGTCCAACG
Reverse: TAGCCAGGTTGCGAAGAACT
Notch1 Forward: AGTCTCTGCAGTGCTGGAAGTA
Reverse: CTTGCAGTACTGGTCGTACAGG
Muc1 (Mucin 1) Forward: CCATTCCACTCCACTCAGGT
Reverse: CCACATGAGCTTCCACACAC
AnkG (Ankyrin G) Forward: CATTCCTCCACGCAAGTGTA
Reverse: GTGGGTTGGCCAGTTTATGT
AQP1 (Aquaporin 1) Forward: CTGCACAGGCTTGCTGTATG
Reverse: TGTTCCTTGGGCTGCAACTA
MGP Forward: GCCGCCTTAGCGGTAGTAAC
Reverse: TCTCTGCTGAGGGGATATGA
CHI3L1 Forward: CCTTGACCGCTTCCTCTGTA
Reverse: GTGTTGAGCATGCCGTAGAG
MYOC (Myocilin) Forward: AAGCCCACCTACCCCTACAC
Reverse: TCCAGTGGCCTAGGCAGTAT
ELAM1 Forward: ACACCTCCACGGAAGCTATG
Reverse: AATTGCAACCAGGTGTGTGTA
MMP1 Forward: TGGACCTGGAGGAAATCTTG
Reverse: AGAATGGCCGAGTTCATGAG
Neurofilament Forward: GAGGAACACCAAGTGGGAGA
Reverse: CTCCTCCTCTTTGGCCTCTT
GFAP Forward: ACTACATCGCCCTCCACATC
Reverse: CAAAGGCACAGTTCCCAGAT
ap2 Forward: CATGGCCAAACCTAACATGA
Reverse: AATTCCTGGCCCAGTATGAA
Leptin Forward: TCCTGGATTCCTTTCCTTCA
Reverse: CAATCGAGGAGGGCAGAATA
KERA (keratocan) Forward: ATCTGCAGCACCTTCACCTT
Reverse: CATTGGAATTGGTGGTTTGA
ALDH Forward: CATTGGCACCTGGAACTACC
Reverse: GGCTTGAGGACCACTGAGTT