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. 2012 Mar 26;53(3):1696–1703. doi: 10.1167/iovs.11-9307

Figure 2.

Figure 2.

Colocalization of Thy1 and HCN4 in ganglion cell somata. (A–C) A single optical section through the ganglion cell layer of a retina incubated in monoclonal anti-HCN4 primary and DyLight 488-conjugated secondary (A, C), anti-Thy1 primary and DyLight 549-conjugated secondary (B, C), and anti-Brn3a primary and DyLight 649-conjugated secondary (C). Fluorescence from DyLight 488, 549, and 649 were assigned to the green, red, and blue color channels, respectively, and are merged in (C). (D–F) Single optical section through the ganglion cell layer of a different portion of the same retina, processed as in (A–C) except that the anti-Thy1 primary was not applied. The scale bar in (C) (20 μm) applies to (C) alone; the scale bar in (F) (20 μm) applies to (A, B, D–F).