FIGURE 2.

The transformation analysis to identify the mutation site important for Δho1R. Left and right panels show the results of the transformation assay with DNA fragments containing sll1512 and slr0887, respectively. DNA fragments amplified from the genome of Δho1 and Δho1R were used in the top and bottom panels, respectively. We confirmed that Δho1 carries the wild-type copies of sll1512 and slr0887. The black dots at the center indicate the points where DNA fragments were spotted (100 ng), and the dashed circles indicate the area where the DNA solutions were spread. The sequences above the pictures show the mutation sites in the Δho1R. Red and green characters correspond to wild-type and Δho1R sequences, respectively.