FIGURE 1.

Diagram of FN and FN constructs, schematic of the F1 adhesin, and sequences of HADD versus FUD. A, each subunit of FN consists of 12 FNI modules (ovals), 2 FNII modules (diamonds), and 15 FNIII modules (squares) for the V89 splice variant shown. In plasma FN, one subunit contains a variable region, and the other subunit lacks it. Modules are numbered to facilitate naming recombinant proteins according to modular content. The boundaries of fibrin- and gelatin-binding domains are indicated, as are locations of epitopes for mAbs (asterisks). B, schematic of the F1 adhesin showing the signal sequence (S), four proline-rich repeats (small rectangles), upstream region (UR), numbered FNBRs, downstream region (DR), the wall-spanning region (W), and membrane-spanning region (M). C, five FNBRs and the upstream and downstream nonrepetitive regions of F1 adhesin (SfbI) and relationship of this sequence to FUD and HADD. The tails introduced into FUD or HADD by the cloning strategy are in lowercase. In HADD, 33 residues of the second or fourth FNBR are joined to 16 residues of the downstream region to add the binding sequence for ¹FNI. The underlined sequences in HADD are predicted to interact with the indicated FNI modules (22, 24, 35).