FIGURE 5.

N-⁹FNI and N-³FNIII compete similarly for FUD or HADD binding to adsorbed FN. Binding of 0.3 nm b-FUD (A) or 0.3 nm b-HADD (B) to coated FN in the presence of increasing concentrations of soluble N-⁹FNI (■), N-³FNII (△), or FN (▴). Assays were in Tris buffer, pH 7.4, containing 50 mm NaCl. Values are expressed relative to biotinylated polypeptide alone and represent mean ± S.D. of three experiments.