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. 2012 Feb 23;287(16):13430–13441. doi: 10.1074/jbc.M112.343798

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© 2012 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 6. — Site-specific contacts of TatC with TatA, TatB, and TatC. UV-induced cross-linking of INV carrying Bpa variants of TatC. Experimental details are as described in the legend for Fig. 5 using the indicated antibodies. A, the adduct (stars) to TatC(I14Bpa) is recognized by αTatB and αTatC, and those to TatC(D63Bpa) are recognized only by αTatC (triangle). B, adducts to TatC(D150Bpa) and TatC(D211Bpa) (top panel) are also recognized by αTatB (middle panel) and αTatA (bottom panel), respectively. TatC(E170Bpa) and TatC(D188Bpa) serve as negative controls that did not give rise to any αTat-reactive cross-link.