FIGURE 2.
Gs-PKA signaling promotes neuronal cell migration. A, neuronal progenitor cells in a Boyden chamber were stimulated with 10 nm PACAP and either 1 μm PACAP6–38 (Antagonist) or 1 μm KT5720 (KT). After 16 h, the number of cells that had migrated into the lower chamber was counted. **, p < 0.005, Student's t test. The S.D. is shown as a bar. n = 4. B, endogenous DCX in neuronal progenitor cells was down-regulated by three shRNAs. Short hairpin Luciferase (shLuc) was used as the control. The number of migrated cells was counted 16 h after treatment with 1 μm Fsk or 1 μm KT5720 (KT). ***, p < 0.001, Student's t test. n = 4. C and D, endogenous DCX in neuronal progenitor cells was down-regulated by sh3′UTR-DCX. After knockdown, cells were infected again with adenoviruses harboring DCX-WT and mutants. Cells were treated with 1 μm forskolin alone or with 1 μm KT5720, and the number of migrated cells was counted after 16 h. n = 7.