FIGURE 4.

PKA signaling decreases DCX-dependent microtubule bundling. A–E, COS7 cells were transfected with EGFP (A), EGFP-DCX-WT (B), EGFP-DCX-S47A (C), EGFP-DCX-S47E (D), or EGFP-DCX-S47R (E) (green). After 2 days cells were treated with 1 μm Fsk either alone or with 10 μm KT5720 (KT). Control cells were treated with 1% DMSO for 1.5 h. Tubulin was immunostained with an anti-tubulin antibody (red). Arrows indicate microtubule bundling. Scale bars, 10 μm. F, the number of cells displaying microtubule bundling was counted, and the percentage of cells exhibiting microtubule bundling among GFP-positive cells was calculated.