Phospho-mimic mutant induces lamellipodium formation in NPCs.
A–E, NPCs were transfected with EGFP-actin and mCherry (A), mCherry-DCX-WT (B), mCherry-DCX-S47A (C), mCherry-DCX-S47E (D), or mCherry-DCX-S47R (E). The dynamics of EGFP-actin and mCherry-DCX were observed by fluorescence microscopy. F, NPCs were transfected with EGFP-actin and a constitutively active (C.A.) form of Rac1. G, NPCs were transfected with EGFP-actin, mCherry-DCX-S47E, and a dominant negative (D.N.) form of Rac1. Scale bars, 10 μm.