TABLE 3.

Kinetics of interaction between inactive and active JNK1β1 with B-F-ATF2 in the absence or presence of ATP

Reported values are representative of a single experiment ± S.E. Similar results were obtained in replicate experiments.

Ligand	Analyte JNK1β1		Rate constants		KD
	Inactive	Active	kon	koff
			m−1s−1	s−1	μm
B-F-ATF2	*		5.85 ± 0.02 × 102a	6.56 ± 0.02 × 10−4b	1.12c
			1.94 ± 0.03 × 104d	5.29 ± 0.05 × 10−3e	0.26f
					2.60 ± 0.34g
B-F-ATF2		*	5.36 ± 0.11 × 102a	2.5 ± 0.01 × 10−3b	4.32c
			2.19 ± 0.09 × 104d	9.45 ± 0.12 × 10−2e	4.66f
					11.0 ± 1.1g
B-F-ATF2, ATP	*		3.21 ± 0.03 × 102a	7.05 ± 0.04 × 10−4b	3.27c
			2.38 ± 0.04 × 104d	5.23 ± 0.05 × 10−2e	2.42f
					0.80 ± 0.04g
B-F-ATF2, ATP		*	7.28 ± 0.16 × 103a	3.54 ± 0.04 × 10−3b	0.63c
			1.39 ± 0.06 × 105d	8.72 ± 0.15 × 10−2e	0.49f
					1.70 ± 0.25g

^a k_on1, derived from the curve fitting of data with the 2:1 HL model.

^b k_off1, derived from the curve fitting of data with the 2:1 HL model.

^c K_D1, derived from the curve fitting of data with the 2:1 HL model.

^d k_on2, derived from the curve fitting of data with the 2:1 HL model.

^e k_off2, derived from the curve fitting of data with the 2:1 HL model.

^f K_D2, derived from the curve fitting of data with the 2:1 HL model.

^g K_D obtained from steady state analysis of the secondary plot Response at equilibrium versus concentration of analyte.