FIGURE 3.

Stopped-flow traces for dCTP incorporation and binding opposite 8-oxoG at 20 °C. A preincubated solution containing Alexa Fluor 594-labeled Dpo4 (600 nm) and either extendable DNA substrate O-2 (A–D) or non-extendable DNA substrate O-3 (E–H) (100 nm) (Table 1) was rapidly mixed with dCTP (1 mm), and the resulting donor and acceptor fluorescence signals upon excitation of the donor at 493 nm were recorded in real-time. Donor and acceptor traces are shown for the finger domain mutant N70C (A and E), the palm domain mutant S112C (B and F), the thumb domain mutant S207C (C and G), and the LF domain mutant K329C (D and H).