Skip to main content
. 2012 Feb 29;287(16):12750–12758. doi: 10.1074/jbc.M111.337907

FIGURE 2.

FIGURE 2.

FOXO expression rescues PINK1 null mutant phenotypes. A, light stereo micrographs of the thoraces of PINK1 null mutants (B9, hs), FOXO-expressing PINK1 null mutants (B9, hs>FOXO), and PINK1 and FOXO double mutants (B9, FOXO−). hs-GAL4/+ (hs) flies were used as wild type controls. White arrows indicate collapsed-thorax phenotypes. B, percentage of defective thorax and wing phenotypes. C, comparison of climbing ability (n = 4). D, toluidine blue-stained longitudinal sections (top panels) and merged images of TUNEL (red) and DAPI (blue) staining (bottom panels) of indirect flight muscle in the thoraces. E, quantification of the mtDNA of thoraces (n = 3). Cox I, cytochrome c oxidase subunit I; Cox III, cytochrome c oxidase subunit III; Cyt B, cytochrome b. F, comparison of the ATP content of thoraces (n = 3). Significance was determined by one-way ANOVA with Bonferroni's correction (*, p < 0.05; **, p < 0.01; ***, p < 0.001; NS, not significant). Error bars indicate mean ± S.D. Scale bars: yellow, 5 μm; white, 10 μm.