Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2012 Feb 27;287(16):12612–12621. doi: 10.1074/jbc.M111.334151

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2012 by The American Society for Biochemistry and Molecular Biology, Inc.

PMC Copyright notice

FIGURE 3. — Subcellular localizations of SIP and AIF. A, subcellular localizations of SIP protein. pEGFP-AIF and DsRed-mito or pEGFP-SIP and DsRed-mito were cotransfected into HeLa cells. EGFP and DsRed fluorescence were visualized by fluorescence microscopy. 4,6-Diamidino-2-phenylindole dihydrochloride (DAPI) staining was also included to visualize the cell nucleus. B, subcellular colocalization of SIP and AIF. HeLa cells were transfected with pEGFP-SIP. Twenty-four hours after transfections, EGFP fluorescence and rhodamine staining of AIF were visualized by fluorescence microscopy. C, analysis of subcellular localization of SIP by subcellular fractionation. HeLa cells were subjected to subcellular fractionation, and immunoblotting was performed with cytoplasm (Cytosol) and mitochondrial (Mito) fractions. Tublin and Tim23 were used as cytosolic and mitochondrial marker proteins, respectively.