FIGURE 5.
Neurotoxicity of PrP peptides on CGN. Dose-dependence of PrP106–126(A117V) fibril neurotoxicity was examined in CGN. The neurons were exposed to different dosages of PrP106–126(A117V) (5 μm to 100 μm) (A) or PrP106–126(A117V) (100 μm) and scrambled control peptide (100 μm) (B) for 3 days. Cell viability was determined by staining neurons with fluorescein diacetate/propidium iodide. Values are expressed as percentages (%) of control (untreated). The data represent the mean ± S.D. (bars) values of triplicate determinations from a single but representative experiment, which has been repeated three times with similar results (**, p < 0.01; ***, p < 0.001 by one-way ANOVA).