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. 2012 Feb 29;287(16):12867–12878. doi: 10.1074/jbc.M111.307249

FIGURE 5.

FIGURE 5.

SIK cooperates with Smurf2 in down-regulation of the ALK5 receptor. A, shown is the immunoblot analysis of CA-ALK5 protein stability in COS-1 cells co-expressing the indicated combinations of wild-type Smurf2 and Smad7 constructs in the absence or presence of two doses of wild-type SIK (triangles). TCL, total cell lysates. B, shown is immunoblot analysis of CA-ALK5 protein levels in COS-1 cells co-transfected with the indicated combinations of wild-type Smurf2 and wild-type or kinase inactive (KR) mutant SIK. C, shown is triple immunofluorescence analysis of wild-type GFP-SIK, CA-ALK5, and catalytically inactive Smurf2(CG) in transfected Mv1Lu cells. Insets show higher magnifications of peripheral punctate structures just below the plasma membrane. The bar represents 10 μm.