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. 2012 Feb 29;287(16):12657–12667. doi: 10.1074/jbc.M112.342725

FIGURE 4.

FIGURE 4.

MurA exists predominantly in a tightly locked state with UNAM and covalently attached PEP. Previous crystal structures of MurA were misinterpreted and actually contain UNAM and the Cys-115-PEP adduct. Structure factors deposited in the PDB were refined against MurA with and without ligands, and the resulting 2 Fo − Fc electron density maps contoured at 1σ are shown for MurA from H. influenzae (PDB entry 3SWE, original 2RL1), which was originally interpreted as UNAG and sulfate ion (inset) (24) (A), MurA from A. aeolicus (PDB entry 3SWG, original 2YVW), which was originally interpreted as EP-UNAG and phosphate ion (B), and MurA from E. coli (PDB entry 3SWD, original 3ISS), which was originally interpreted as UNAM and phosphite ion (25) (C). The Fo − Fc electron density maps omitting the ligands during the refinement are shown in supplemental Fig. S2.